Total Solution for On-Site Diagnosis of ASF
 ASF Fast POCT Kit |
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Palm PCR™ ASFV Direct Prep Kit for ultra-fast ≤ 15 min direct preparation of ASFV DNA from plasma and serum samples of suspected pigs |
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Palm PCR™ ASFV Fast PCR Kit for ultra-fast ≤ 25 min PCR and real-time detection |
| Fully-Mobile Equipment |
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Palm PCR™ S1/S1e Ultra-fast Mobile Real-time PCR System for ultra-fast ≤ 25 min hot-start real-time PCR using Palm PCR™ ASFV Fast PCR Kit |
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Ahram™ RX1 Portable Centrifuge for on-site preparation of blood samples and real-time PCR mix |
Simple, Convenient & Fast Workflow
| Step 1. ASFV Direct Prep |
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Add 20 μL of a plasma or serum sample into an ASFV Direct Prep tube. |
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Perform 2-step isothermal incubation on Palm PCR™ S1/S1e device. |
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Centrifuge the incubated Direct Prep tube for 2 min at 8,500 RPM on Ahram™ RX1 Portable Centrifuge, and use 5 μL of the clear
supernatant for real-time PCR diagnosis. |
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| Step 2. ASFV Real-time PCR Diagnosis |
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Add 5 μL of the clear supernatant into a 0.2 mL tube containing 17 μL premix of Palm PCR™ ASFV Fast PCR Kit, and mix well. |
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Transfer all 22 μL of the mixed solution into a Type IIA Palm PCR™ sample tube and close with an optical cap. |
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Centrifuge the Palm PCR™ sample tube for 2 min at 8,500 RPM on Ahram™ RX1 Portable Centrifuge, and perform hot-start real-time PCR on Palm PCR™ S1/S1e device. |
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Ultra-Fast MDx with Extreme Sensitivity
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Sensitivity of the ASF Fast POCT System
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| Fast POCT diagnosis of ASF using the Direct Prep method can deliver accurate results in a short turnaround time (within 40 min) with very high sensitivity that is equivalent to typical DNA extraction methods. |
Sensitivity of the Direct Prep method is tested using the URL-ASF reference sample (ASFV positive serum) obtained from the European Union Reference Laboratory for ASF (EURL-ASF), CISA-INIA, Spain. The URL-ASF reference sample was serially diluted with an ASFV negative serum and each diluted serum sample was processed with Palm PCR™ ASFV Direct Prep Kit. The processed sample was tested using Palm PCR™ ASF Fast POCT Kit on Palm PCR™ S1/S1e device.
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| Shown on the right are results of the ASF Fast POCT diagnosis using the Direct Prep method. As shown, the ASF Fast POCT diagnosis successfully detect ASFV DNA at 1/10,000 dilution with 100% hit rate, and at 1/100,000 dilution (about 2.5 copies/reaction) with about 70% hit rate. Serial dilutions with an ASFV negative plasma also delivered identical results (data not shown). | |
